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ER beta/NR3A2 Antibody (ESR2/686)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44366

Novus Biologicals, part of Bio-Techne
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NBP2-44366-0.02mg
NBP2-44366-0.1mg

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # ESR2/686

Concentration

0.2 mg/ml

Product Specifications

Immunogen

C-terminus fragment of recombinant human ER beta/NR3A2 protein (Uniprot: Q92731)

Localization

Predominantly nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47797)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ER beta/NR3A2 Antibody (ESR2/686)

Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]

Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]

Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).
Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]

Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]

Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ESR2/686).
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]

Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366]

Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) [NBP2-44366] - Human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).

Applications for ER beta/NR3A2 Antibody (ESR2/686)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ER beta/NR3A2

The human ER-

Long Name

Estrogen Receptor beta

Alternate Names

ESR2, NR3A2

Gene Symbol

ESR2

UniProt

Additional ER beta/NR3A2 Products

Product Documents for ER beta/NR3A2 Antibody (ESR2/686)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ER beta/NR3A2 Antibody (ESR2/686)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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