ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47797
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]](https://resources.bio-techne.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47797-img0005.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]](https://resources.bio-techne.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47797-img0007.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # ESR2/686
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
C-terminus fragment of recombinant human ER beta/NR3A2 protein (Uniprot: Q92731)
Localization
Predominantly nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44366).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).![Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]](https://resources.bio-techne.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47797-img0002.jpg "Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]")
Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]
Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Antibody (ESR2/686).![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]](https://resources.bio-techne.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47797-img0004.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]")
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]
Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]](https://resources.bio-techne.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47797-img0006.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]")
![Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]](https://resources.bio-techne.com/images/products/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47797-img0001.jpg "Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797]")
Applications for ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ER beta/NR3A2
Long Name
Estrogen Receptor beta
Alternate Names
ESR2, NR3A2
Gene Symbol
ESR2
Additional ER beta/NR3A2 Products
Product Documents for ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free
Product Specific Notices for ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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