ERCC1 Antibody (CL1249) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43959

Novus Biologicals, part of Bio-Techne
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NBP3-43959
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Catalog #
BSA Free
NBP2-34479
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL1249

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ERCC1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07992, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for ERCC1 Antibody (CL1249) - Azide and BSA Free

ERCC1 Antibody (CL1249) - Azide and BSA Free Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43959]

Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43959]

Staining of human breast cancer shows moderate nuclear positivity in tumor cells.
ERCC1 Antibody (CL1249) - Azide and BSA Free Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43959]

Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43959]

Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
ERCC1 Antibody (CL1249) - Azide and BSA Free Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43959]

Immunohistochemistry-Paraffin: ERCC1 Antibody [NBP3-43959]

Staining of human prostate cancer shows moderate nuclear positivity in tumor cells.
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Applications for ERCC1 Antibody (CL1249) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (CL1249) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ERCC1 Antibody (CL1249) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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