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Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81097JF525

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-81097JF525

Key Product Details

Species Reactivity

Validated:

Human

Applications

Competitive ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Janelia Fluor 525

Antibody Source

Recombinant Monoclonal Human IgG1 kappa Clone # H22

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525]

Immunogen

This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human Fc gamma RI/CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.

Specificity

This immunotoxin is specific for human Fc gamma RI/CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences

Clonality

Monoclonal

Host

Human

Isotype

IgG1 kappa

Applications for Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525]

Application
Recommended Usage

Competitive ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fc gamma RI/CD64

CD64 is a 72 kD single chain type I glycoprotein also known as Fc gammaRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN- gamma stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Long Name

Fc gamma Receptor I

Alternate Names

CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA

Gene Symbol

FCGR1A

Additional Fc gamma RI/CD64 Products

Product Documents for Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 525]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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