Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81098
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free [NBP2-81098]](https://resources.bio-techne.com/images/products/Fc-gamma-RI-CD64-Antibody-H22-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81098-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free [NBP2-81098]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Competitive ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # H22
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free
Immunogen
This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human Fc gamma RI/CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.
Specificity
This immunotoxin is specific for human Fc gamma RI/CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free [NBP2-81098]
Immunocytochemistry/Immunofluorescence: Fc gamma RI/CD64 Antibody (H22) - Chimeric [NBP2-81098] - Immunofluorescence staining of fixed U937 cells with Fc gamma RI/CD64 Antibody (H22) [NBP2-81098]. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of H22 [NBP2-81098] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81098, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.Applications for Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Competitive ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds human CD64 and can be bound to a toxin so that together it displays specific cytotoxicity, and thus can be used to selectively eliminate AML cells; FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur, 2003). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur, 2011).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Fc gamma RI/CD64
Long Name
Fc gamma Receptor I
Alternate Names
CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA
Gene Symbol
FCGR1A
Additional Fc gamma RI/CD64 Products
Product Documents for Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free
Product Specific Notices for Fc gamma RI/CD64 Antibody (H22) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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