Fc gamma RI/CD64 Antibody (H22) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81098CL1
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Competitive ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
CoraFluor 1
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # H22
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Fc gamma RI/CD64 Antibody (H22) [CoraFluor™ 1]
Immunogen
This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human Fc gamma RI/CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.
Specificity
This immunotoxin is specific for human Fc gamma RI/CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Fc gamma RI/CD64 Antibody (H22) [CoraFluor™ 1]
Application
Recommended Usage
Competitive ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Fc gamma RI/CD64
Long Name
Fc gamma Receptor I
Alternate Names
CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA
Gene Symbol
FCGR1A
Additional Fc gamma RI/CD64 Products
Product Documents for Fc gamma RI/CD64 Antibody (H22) [CoraFluor™ 1]
Product Specific Notices for Fc gamma RI/CD64 Antibody (H22) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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