FE65 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58360

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.3 mg/ml

View all FE65 Antibodies »

Product Specifications

Immunogen

Recombinant rat FE65, expressed in bacteria. [Swiss-Prot# P46933]

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FE65 Antibody - BSA Free

Western Blot: FE65 AntibodyBSA Free [NB110-58360]

Western Blot: FE65 AntibodyBSA Free [NB110-58360]

Western Blot: FE65 Antibody [NB110-58360] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY APBB1 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APBB1.
Western Blot: FE65 AntibodyBSA Free [NB110-58360]

Western Blot: FE65 AntibodyBSA Free [NB110-58360]

Western Blot: FE65 Antibody [NB110-58360] - Protein on HEK 293 cell lysate.

Applications for FE65 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug

Western Blot

1:1000
Application Notes
This FE65 antibody is useful in Western blot and Immunoprecipitation. In Western blot a band is seen at ~100 kDa versus the predicted molecular weight of 77 kDa, which corresponds to earlier findings in literature (PMID: 9685356). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 15% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FE65

Fe65 (Protein Fe65 or APBB1) is a neuronal adaptor protein that mediates the assembly of multimolecular complexes through a variety of protein-protein interaction domains: the WW domain, which binds to proline-rich sequences, and two C-terminal phosphotyrosine-binding domains. FE65 is primarily found in CNS and is highly expressed in neurons of hippocampus, cerebellum, thalamus, and brain stem nuclei. Fe65 is localized within cytoplasm or nucleus, although its moderate amounts are tethered to membranes via binding with Alzheimer's disease amyloid precursor protein (APP). FE65 forms stable, transcriptionally active complex with AICD (APP intracellular domain) that follows nuclear translocation of AICD and apoptosis through Tip60. FE65 is functionally linked to cellular motility, morphology and actin dynamics through binding of its WW domain to actin-binding protein Mena. It plays a key role in response to DNA damage by translocating to nucleus and inducing apoptosis through recognizing/binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at DSBs, recruiting other pro-apoptosis factors such as MAPK8/JNK1. It is required for histone H4 acetylation DSBs and its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. It functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor that inhibits the expression of CASP4.

Alternate Names

adaptor protein FE65a2, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), amyloid beta A4 precursor protein-binding, family B, member 1, Fe65, FE65amyloid beta A4 precursor protein-binding family B member 1, MGC:9072, Protein Fe65, RIR, stat-like protein

Gene Symbol

APBB1

Additional FE65 Products

Product Documents for FE65 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FE65 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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