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Feline IL-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF984

R&D Systems, part of Bio-Techne
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AF984
AF984-SP

Key Product Details

Species Reactivity

Feline

Applications

Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant feline IL-4
Gln24-His133
Accession # P55030

Specificity

Detects feline IL‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant canine IL-4, recombinant cotton rat IL-4, recombinant human IL-4, recombinant mouse IL-4, recombinant porcine IL-4, and recombinant rat IL-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Feline IL-4 Antibody

Cell Proliferation Induced by IL-4 and Neutralization by Feline IL-4 Antibody.

Cell Proliferation Induced by IL-4 and Neutralization by Feline IL-4 Antibody.

Recombinant Feline IL-4 (Catalog # 984-FL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF984). The ND50 is typically 2-8 µg/mL.

Applications for Feline IL-4 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Feline IL-4 (Catalog # 984-FL)

Neutralization

Measured by its ability to neutralize IL-4-induced proliferation in the TF-1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 100 ng/mL Recombinant Feline IL-4.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure (4). Feline IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature feline IL-4 shares 81%, 64%, 49%, 40%, and 40% aa sequence identity with canine, bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on feline dendritic cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8-11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

References

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. SwissProt # P55030.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Sprague, W.S. et al. (2005) J. Comp. Pathol. 133:136.
  6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Gene Symbol

IL4

UniProt

Additional IL-4 Products

Product Documents for Feline IL-4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Feline IL-4 Antibody

For research use only

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