FGF acidic/FGF1 Antibody (3X3E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16609

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16609-100ul
NBP3-16609-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3X3E5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FGF acidic/FGF1 Antibodies »

Product Summary for FGF acidic/FGF1 Antibody (3X3E5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 56-155 of human FGF acidic/FGF1 (P05230). HIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGF acidic/FGF1 Antibody (3X3E5)

Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]

Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]

Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Western blot analysis of extracts of various cell lines, using FGF acidic/FGF1 Rabbit mAb (NBP3-16609) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]

Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Immunofluorescence analysis of U-2 OS cells using FGF acidic/FGF1 Rabbit mAb (NBP3-16609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]

Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Immunofluorescence analysis of C6 cells using FGF acidic/FGF1 Rabbit mAb (NBP3-16609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for FGF acidic/FGF1 Antibody (3X3E5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF acidic is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF acidic can translocate to the cytosol and to the nucleus. Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.

Long Name

Fibroblast Growth Factor acidic

Alternate Names

alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1

Gene Symbol

FGF1

Additional FGF acidic/FGF1 Products

Product Documents for FGF acidic/FGF1 Antibody (3X3E5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FGF acidic/FGF1 Antibody (3X3E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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