FGFR1 Antibody (M2F12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-829

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # M2F12

Format

BSA Free

Concentration

1.0 mg/ml

View all FGFR1 Antibodies »

Product Specifications

Immunogen

Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

Epitope

NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.

Specificity

Reacts with alpha isoform of FRFR1. Reacts with the NH2-terminus of unique NH2-terminal Ig loop of FGFr1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for FGFR1 Antibody (M2F12) - BSA Free

Western Blot: FGFR1 Antibody (M2F12)BSA Free [NB120-829]

Western Blot: FGFR1 Antibody (M2F12)BSA Free [NB120-829]

Western Blot: FGF R1 Antibody (M2F12) [NB120-829] - Total protein from Ntera-2 was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/ml of anti-FGFR1. FGFR1 protein was detected at approximately 150 kDa using an anti-mouse HRP secondary antibody.
Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Immunocytochemistry/Immunofluorescence: FGF R1 Antibody (M2F12) [NB120-829] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with FGR1 (M2F12) (NB120-829) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Flow Cytometry: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Flow Cytometry: FGFR1 Antibody (M2F12) - BSA Free [NB120-829]

Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in MCF-7 cells (1 x 10^6 cells/ml) stained with FGF r1 antibody (NB120-829, red peaks) at a 1:1000 dilution. Detected with a FITC conjugated goat anti-mouse IgG. Shown with mIgG2a isotype control (green). Two distinct samples shown.
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Applications for FGFR1 Antibody (M2F12) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

reported in scientific literature (PMID 8168112)

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/mL
Application Notes
Western blotting: 1 ug/mL recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1. Immunoprecipitation: Native, nuclear, recombinant, and ligand-labeled FGFr1. Customers have reported difficulties using this antibody in immunocytochemistry staining (see review). This antibody does not appear to work in IHC paraffin embedded sections.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 14182 (Mouse); 79114 (Rat)

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (M2F12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FGFR1 Antibody (M2F12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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