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FGFR3 Antibody (HL1595) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25465

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25465

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Canine, Feline

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1595

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FGFR3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGFR3 Antibody (HL1595) - Azide and BSA Free

FGFR3 Antibody (HL1595) - Azide and BSA Free

Western Blot: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] -

Western Blot: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FGFR3 antibody [HL1595] (NBP3-25465) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FGFR3 Antibody (HL1595) - Azide and BSA Free

Knockout Validated: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] -

Knockout Validated: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] - Wild-type (WT) and FGFR3 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR3 antibody [HL1595] (NBP3-25465) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
FGFR3 Antibody (HL1595) - Azide and BSA Free

Western Blot: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] -

Western Blot: FGFR3 Antibody (HL1595) - Azide and BSA Free [NBP3-25465] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FGFR3 antibody [HL1595] (NBP3-25465) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for FGFR3 Antibody (HL1595) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR3

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Gene Symbol

FGFR3

Additional FGFR3 Products

Product Documents for FGFR3 Antibody (HL1595) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FGFR3 Antibody (HL1595) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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