Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B10

Concentration

1 mg/ml

View all FGFR3 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 23-375 of human FGFR3 (NP_000133) produced in SF9 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

85.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FGFR3 Antibody (OTI1B10)

Western Blot: FGFR3 Antibody (OTI1B10) [NBP2-45716]

Western Blot: FGFR3 Antibody (OTI1B10) [NBP2-45716]

Western Blot: FGFR3 Antibody (OTI1B10) [NBP2-45716] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells and FGFR3-Knockout 293T cells were separated by SDS-PAGE and immunoblotted with anti-FGFR3 monoclonal antibody TA801078. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody as a loading control (1:500).
Immunohistochemistry: FGFR3 Antibody (OTI1B10) [NBP2-45716]

Immunohistochemistry: FGFR3 Antibody (OTI1B10) [NBP2-45716]

Immunohistochemistry: FGFR3 Antibody (OTI1B10) [NBP2-45716] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: FGFR3 Antibody (OTI1B10) [NBP2-45716]

Western Blot: FGFR3 Antibody (OTI1B10) [NBP2-45716]

Western Blot: FGFR3 Antibody (OTI1B10) [NBP2-45716] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR3.

Applications for FGFR3 Antibody (OTI1B10)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGFR3

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Gene Symbol

FGFR3

Additional FGFR3 Products

Product Documents for FGFR3 Antibody (OTI1B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FGFR3 Antibody (OTI1B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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