FITC Antibody (SPM395) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08492
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM395
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Fluorescein isothiocyanate (FITC) conjugated to KLH
Localization
Depends on the target
Specificity
This monoclonal antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this monoclonal antibody is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07778).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for FITC Antibody (SPM395) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Pretreatment depends upon the fluoreceinated primary antibody. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: FITC
Long Name
Fluorescein Isothiocyanate
Alternate Names
Fluorescein Isothiocyanate
Additional FITC Products
Product Documents for FITC Antibody (SPM395) - Azide and BSA Free
Product Specific Notices for FITC Antibody (SPM395) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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