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FITC Antibody (SPM395) [mFluor Violet 450 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08492MFV450

Novus Biologicals, part of Bio-Techne
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NBP3-08492MFV450

Key Product Details

Species Reactivity

Non-species specific

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In-situ Hybridization, Western Blot

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM395

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Fluorescein isothiocyanate (FITC) conjugated to KLH

Localization

Depends on the target

Specificity

This monoclonal antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this monoclonal antibody is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for FITC Antibody (SPM395) [mFluor Violet 450 SE]

FITC Antibody (SPM395) [mFluor Violet 450 SE] [NBP3-08492MFV450] -

FITC Antibody (SPM395) [mFluor Violet 450 SE] [NBP3-08492MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.

Applications for FITC Antibody (SPM395) [mFluor Violet 450 SE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

In-situ Hybridization

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FITC

FITC is a small organic fluorochrome dye commonly used for a variety of labeling applications such as conjugation to antibodies or other proteins. Excitation of FITC results in emission of a visible yellow-green signal.

Long Name

Fluorescein Isothiocyanate

Alternate Names

Fluorescein Isothiocyanate

Additional FITC Products

Product Documents for FITC Antibody (SPM395) [mFluor Violet 450 SE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FITC Antibody (SPM395) [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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