FITC Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64617

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all FITC Antibodies »

Product Specifications

Immunogen

Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)

Specificity

Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for FITC Antibody - Azide and BSA Free

Fluorophore-linked immunosorbent assay: FITC Antibody - Azide and BSA Free [NB100-64617]

Fluorophore-linked immunosorbent assay: FITC Antibody - Azide and BSA Free [NB100-64617]

Fluorophore-linked immunosorbent assay: FITC Antibody [NB100-64617] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE. Image using the Alkaline Phosphatase form of this antibody.

Applications for FITC Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Western Blot

1:100-1:2000
Application Notes
WB reactivity reported in (PMID: 24166503).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FITC

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Long Name

Fluorescein Isothiocyanate

Alternate Names

Fluorescein Isothiocyanate

Additional FITC Products

Product Documents for FITC Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FITC Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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