FITC Antibody [Alkaline Phosphatase]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64618

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Western Blot

Cited:

Western Blot

Label

Alkaline Phosphatase

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FITC Antibodies »

Product Specifications

Immunogen

Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)

Specificity

Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for FITC Antibody [Alkaline Phosphatase]

Fluorophore-linked immunosorbent assay: FITC Antibody [Alkaline Phosphatase] [NB100-64618] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE.

Applications for FITC Antibody [Alkaline Phosphatase]

Application
Recommended Usage

ELISA

1:2000-1:4000

Fluorophore-linked immunosorbent assay

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
WB reactivity reported in (PMID: 24166503).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50 mM Tris (pH 8.0), 1 mM MgCl2, and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FITC

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Long Name

Fluorescein Isothiocyanate

Alternate Names

Fluorescein Isothiocyanate

Additional FITC Products

Product Documents for FITC Antibody [Alkaline Phosphatase]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FITC Antibody [Alkaline Phosphatase]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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