Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80739
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Lambda Clone # 7E8.2C8
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Partial recombinant human FLT3 protein (between amino acids 250-500) [UniProt P36888]
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: ~86% to Mouse and Rat's FLT3 protein.
Localization
Endoplasmic reticulum lumen, Membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Lambda
Scientific Data Images for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8)Azide and BSA Free [NBP2-80739]
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position. Image from the standard format of this antibody.Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]
Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells wFlow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis Image from the standard format of this antibody.Applications for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
2 ug/million cells
Immunohistochemistry
5-15 ug/ml
Immunohistochemistry-Paraffin
5-15 ug/ml
Western Blot
2 ug/ml
Application Notes
FLT3 is a 993 amino acids long protein with predicted molecular weight of 112.9 kDa, however, it undergoes extensive glycosylation and phosphorylation so that the mature protein will have relatively higher molecular weights. In WB assay, the glycosylated protein species may run between 113-160 kDa position, while the phosphorylated protein may result on generation of a doublet band.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Flt-3/Flk-2
Long Name
fms-like Tyrosine Kinase 3
Alternate Names
CD135, Flk-2, Flt3, STK-1
Gene Symbol
FLT3
Additional Flt-3/Flk-2 Products
Product Documents for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
Product Specific Notices for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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