Fluorescein Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB120-6656
Key Product Details
Species Reactivity
Non-species specific
Applications
Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fluorescein conjugated to Goat IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
Fluorescein Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum and Fluorescein conjugated IgG.
Fluorescein Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum and Fluorescein conjugated IgG.
Scientific Data Images for Fluorescein Antibody [HRP]
Dot Blot: Fluorescein Antibody [HRP] [NB120-6656]
![Fluorescein Antibody [HRP]](https://resources.bio-techne.com/images/products/nb120-6656_goat-polyclonal-fluorescein-antibody-hrp-235202317492111.jpg "Fluorescein Antibody [HRP]")
Fluorescein Antibody [HRP]
Dot Blot Results of Fluorescein Antibody [HRP]. Blot row top (T): FITC-BSA conjugated. Blot row bottom (B): BSA alone. Load: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Fluorescein Antibody HRP at 1ug/mL in 1hr RT. Imaged with BioRad ChemiDoc, DyLight 549 filter.Applications for Fluorescein Antibody [HRP]
Application
Recommended Usage
ELISA
1:200000
Immunohistochemistry
1:500-1:2500
Immunohistochemistry-Paraffin
1:500-1:2500
Western Blot
1:5000-1:10000
Application Notes
This product has been tested by ELISA, dot blot, and western blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and in situ hybridization, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fluorescein
Alternate Names
FITC, fluorescein isothyocianate
Additional Fluorescein Products
Product Specific Notices for Fluorescein Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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