Fluorescein Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-493
Key Product Details
Species Reactivity
Validated:
Non-species specific
Cited:
Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fluorescein conjugated to Goat IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Fluorescein Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein conjugated Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.
Store vial at 4C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Fluorescein Antibody
![Western Blot: Fluorescein Antibody [NB600-493]](https://resources.bio-techne.com/images/products/Fluorescein-Antibody-Western-Blot-NB600-493-img0004.jpg "Western Blot: Fluorescein Antibody [NB600-493]")
Western Blot: Fluorescein Antibody [NB600-493]
Western Blot: Fluorescein Antibody [NB600-493] - Lane 1: BSA conjugated Fluoresccein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fluorescein primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 67 kDa, 67 kDa for Fluorescein. Other band(s): None.![Western Blot: Fluorescein Antibody [NB600-493]](https://resources.bio-techne.com/images/products/Fluorescein-Antibody-Western-Blot-NB600-493-img0003.jpg "Western Blot: Fluorescein Antibody [NB600-493]")
Western Blot: Fluorescein Antibody [NB600-493]
Western Blot: Fluorescein Antibody [NB600-493] - Analysis using the Biotin conjugate of NB600-493. Detection of Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1000 for 60 min at RT.
Fluorescein Antibody
Western Blot of Fluorescein Antibody. Lane 1: Opal Prestained Molecular Weight MarkerApplications for Fluorescein Antibody
Application
Recommended Usage
ELISA
1:5000-1:25000
Immunohistochemistry
1:250-1:1500
Immunohistochemistry-Paraffin
1:10 - 1:500
Western Blot
1:500-1:3000
Application Notes
This product has been tested by ELISA and western blot and is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Use in IHC-FrFl reported in scientific literature (PMID:35411060)
Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 30228102).
Use in IHC-FrFl reported in scientific literature (PMID:35411060)
Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 30228102).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fluorescein
Alternate Names
FITC, fluorescein isothyocianate
Additional Fluorescein Products
Product Documents for Fluorescein Antibody
Product Specific Notices for Fluorescein Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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