FSH beta Antibody (OTI2A6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70781

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FSH beta Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FSHB (NP_001018090) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FSH beta Antibody (OTI2A6) - Azide and BSA Free

Western Blot: FSH beta Antibody (OTI2A6)Azide and BSA Free [NBP2-70781]

Western Blot: FSH beta Antibody (OTI2A6)Azide and BSA Free [NBP2-70781]

Western Blot: FSH beta Antibody (OTI2A6) - Azide and BSA Free [NBP2-70781] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FSH beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FSH beta.
Immunocytochemistry/ Immunofluorescence: FSH beta Antibody (OTI2A6) - Azide and BSA Free [NBP2-70781]

Immunocytochemistry/ Immunofluorescence: FSH beta Antibody (OTI2A6) - Azide and BSA Free [NBP2-70781]

Immunocytochemistry/Immunofluorescence: FSH beta Antibody (OTI2A6) - Azide and BSA Free [NBP2-70781] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FSH beta.
Flow Cytometry: FSH beta Antibody (OTI2A6) - Azide and BSA Free [NBP2-70781]

Flow Cytometry: FSH beta Antibody (OTI2A6) - Azide and BSA Free [NBP2-70781]

Flow Cytometry: FSH beta Antibody (OTI2A6) - Azide and BSA Free [NBP2-70781] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FSH beta antibody, and then analyzed by flow cytometry.

Applications for FSH beta Antibody (OTI2A6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FSH beta

FSH is a pituitary hormone involved in the maturation of ovarian follicles and estrogen secretion in females. In the pituitary gland, FSH is produced by gonadotrophs. In males, FSH stimulates the secretion of testosterone. Follicle stimulating hormone enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell proliferation and maintenance of sperm quality in the testis. Members of the pituitary glycoprotein hormone family, of which FSH is one (see also luteinizing hormone, chorionic gonadotropin, and thyroid stimulating hormone), consist of a shared alpha chain and a beta chain encoded by a separate gene.

Long Name

Follicle Stimulating Hormone, beta

Alternate Names

Follitropin, FSHB

Gene Symbol

FSHB

Additional FSH beta Products

Product Documents for FSH beta Antibody (OTI2A6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FSH beta Antibody (OTI2A6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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