FXR/NR1H4 Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB400-153V

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FXR/NR1H4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to the C-terminus of the human FXR protein. [UniProt# Q96RI1]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29491146).

Localization

Cytoplasmic, nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for FXR/NR1H4 Antibody [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: FXR/NR1H4

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR is mainly expressed in liver, intestine, kidney, and adrenal glands. FXR heterodimerizes with retinoid X receptor (RXR alpha/NR2B1), associates with FXREs (FXR response elements) that are usually but not exclusively inverted repeat-1 (IR-1), and regulates the transcription of its target genes. FXR interacts with CARM1, SMARD1 and after activation by agonist binding, it interacts with a coactivator, NCOA1 or NCOA2. FXR has been shown to repress transcription of CYP7A1 (cholesterol 7-alpha-hydroxylase gene) through induction of NR0B2 or FGF19 expression, via two distinct mechanisms. FXR activates the intestinal bile acid-binding protein (IBABP) and transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. Altogether, FXR is critical for maintaining a homeostasis in bile acid, cholesterol, lipoprotein, and triglyceride metabolism.

Long Name

Farnesoid X-activated receptor

Alternate Names

BAR, HRR-1, NR1H4, RIP14

Gene Symbol

NR1H4

Additional FXR/NR1H4 Products

Product Documents for FXR/NR1H4 Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FXR/NR1H4 Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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