GABA-AR alpha 4 Antibody (S398A-34)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59326
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S398A-34
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
Localization
Cell Junction , Synapse , Postsynaptic Cell Membrane , Cell Membrane
Specificity
Detects 60kDa. Does not cross-react with other GABA-A-Receptor Alpha subunits.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for GABA-AR alpha 4 Antibody (S398A-34)
Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]
Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]
Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody (NBP2-59326) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.Immunocytochemistry/ Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326]
Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34. (Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT.Applications for GABA-AR alpha 4 Antibody (S398A-34)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: GABA-A R alpha 4
Long Name
gamma-Aminobutyric Acid Type A Receptor, alpha-4 Polypeptide
Alternate Names
GABAAR alpha 4, GABAARa4, GABRA4
Gene Symbol
GABRA4
Additional GABA-A R alpha 4 Products
Product Documents for GABA-AR alpha 4 Antibody (S398A-34)
Product Specific Notices for GABA-AR alpha 4 Antibody (S398A-34)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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