GABA-AR alpha 4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18844
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-EKAKRKTSKPPQE corresponding to internal region according to NP_000800.2.
Epitope
internal region
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GABA-AR alpha 4 Antibody
![Western Blot: GABA-AR alpha 4 Antibody [NBP1-18844]](https://resources.bio-techne.com/images/products/GABA-A-R-alpha-4-Antibody-Western-Blot-NBP1-18844-img0005.jpg "Western Blot: GABA-AR alpha 4 Antibody [NBP1-18844]")
Western Blot: GABA-AR alpha 4 Antibody [NBP1-18844]
Western Blot: GABA-A R alpha 4 Antibody [NBP1-18844] - Analysis of GABA A Receptor alpha 4 in Human Frontal Cortex lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: GABA-AR alpha 4 Antibody [NBP1-18844]](https://resources.bio-techne.com/images/products/GABA-A-R-alpha-4-Antibody-Immunohistochemistry-Paraffin-NBP1-18844-img0004.jpg "Immunohistochemistry-Paraffin: GABA-AR alpha 4 Antibody [NBP1-18844]")
Immunohistochemistry-Paraffin: GABA-AR alpha 4 Antibody [NBP1-18844]
Immunohistochemistry-Paraffin: GABA-A R alpha 4 Antibody [NBP1-18844] - (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for GABA-AR alpha 4 Antibody
Application
Recommended Usage
Immunohistochemistry
2 - 4 ug/ml
Immunohistochemistry-Paraffin
2 - 4 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 61.6 kDa band according to NP_000800.2). IHC-P: Human cortex shows patchy staining near or at the neuronal cell membrane.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GABA-A R alpha 4
Long Name
gamma-Aminobutyric Acid Type A Receptor, alpha-4 Polypeptide
Alternate Names
GABAAR alpha 4, GABAARa4, GABRA4
Entrez Gene IDs
2557 (Human)
Gene Symbol
GABRA4
UniProt
Additional GABA-A R alpha 4 Products
Product Documents for GABA-AR alpha 4 Antibody
Product Specific Notices for GABA-AR alpha 4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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