Galectin-3 Antibody (M3/38) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43313

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # M3/38

Format

BSA Free

Concentration

0.5 mg/ml

View all Galectin-3 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was Galectin3 Mac2.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Galectin-3 Antibody (M3/38) - BSA Free

Flow (Intracellular): Galectin-3 Antibody (M3/38) - BSA Free [NBP1-43313]

Flow (Intracellular): Galectin-3 Antibody (M3/38) - BSA Free [NBP1-43313]

Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.. Using the Biotin format of this antibody.
Flow (Intracellular): Galectin-3 Antibody (M3/38) - BSA Free [NBP1-43313]

Flow (Intracellular): Galectin-3 Antibody (M3/38) - BSA Free [NBP1-43313]

Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - An intracellular stain was performed on RAW 246.7 cells with Galectin-3 Antibody (M3/38) NBP1-43313AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
Flow (Intracellular): Galectin-3 Antibody (M3/38) - BSA Free [NBP1-43313]

Flow (Intracellular): Galectin-3 Antibody (M3/38) - BSA Free [NBP1-43313]

Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis. Image using the Biotin format of this antibody.

Applications for Galectin-3 Antibody (M3/38) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This M3/38 antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Galectin-3

The M3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. This monoclonal antibody M3/38 crossreacts to human Galectin-3 (LGALS3).

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody (M3/38) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Galectin-3 Antibody (M3/38) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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