Flow Cytometry: Galectin-3 Antibody (M3/38) [Biotin] [NBP1-43553] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.

Applications for Galectin-3 Antibody (M3/38) [Biotin]

Application

Recommended Usage

Flow Cytometry

1:10-1:1000

Application Notes

This M3/38 antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Galectin-3

The M3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. This monoclonal antibody M3/38 crossreacts to human Galectin-3 (LGALS3).

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody (M3/38) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Galectin-3 Antibody (M3/38) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Galectin-3 Antibody (M3/38) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43553

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

View all Galectin-3 Antibodies »

Product Summary for Galectin-3 Antibody (M3/38) [Biotin]

Immunogen

Clonality

Host

Isotype

Scientific Data Images for Galectin-3 Antibody (M3/38) [Biotin]

Applications for Galectin-3 Antibody (M3/38) [Biotin]

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Galectin-3

Alternate Names

Gene Symbol

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody (M3/38) [Biotin]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Galectin-3 Antibody (M3/38) [Biotin]

CONTACT INFORMATION

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