Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E3

Concentration

1 mg/ml

View all GAP-43 Antibodies »

Product Specifications

Immunogen

C-terminal peptide of rat and mouse GAP43 (KEDPEADQEHA) to which an N-terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.

Reactivity Notes

Mouse and rat.

Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection > growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction > synapse. Cell projection > filopodium membrane; Peripheral membrane protein. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAP-43 Antibody (1E3)

Western Blot: GAP-43 Antibody (1E3) [NBP1-92713]

Western Blot: GAP-43 Antibody (1E3) [NBP1-92713]

Western Blot: GAP-43 Antibody (1E3) [NBP1-92713] - Western blot of whole rat spinal cord lysates probed with GAP-43 antibody (1E3) antibody to GAP-43 in lane 16 and another similar antibody in lane 17. Dots in middle of strips indicate position of 50kDa and 37kDa protein bands. The strong single band running at about 43kDa corresponds to GAP-43.
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (1E3) [NBP1-92713]

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody (1E3) [NBP1-92713]

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (1E3) [NBP1-92713] - Mixed neuronal cultures stained with GAP-43 antibody (1E3) (green), a rabbit antibody to microtubule associated protein 2 (red) and DNA (blue). The GAP-43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.

Applications for GAP-43 Antibody (1E3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:10000
Application Notes
This GAP43 (1E3) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 43 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NBP1-92713 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAP-43

GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody (1E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GAP-43 Antibody (1E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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