Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GAP-43 Antibodies »

Product Specifications

Immunogen

C-terminal peptide of rat and mouse GAP43 (KEDPEADQEHA) to which an N-terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.

Reactivity Notes

Mouse and rat.

Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection > growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction > synapse. Cell projection > filopodium membrane; Peripheral membrane protein. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Marker

Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for GAP-43 Antibody (1E3) [DyLight 650]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GAP-43

GAP43 (Growth associated protein 43) is a crucial component for regenerative response in the nervous system. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The protein is present at high levels in neuronal growth cones during development and axonal regeneration.

Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody (1E3) [DyLight 650]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GAP-43 Antibody (1E3) [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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