GATA-3 Antibody (GATA3/4550R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13922

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # GATA3/4550R

Concentration

0.2 mg/ml

View all GATA-3 Antibodies »

Product Summary for GATA-3 Antibody (GATA3/4550R)

Immunogen

Recombinant full-length human GATA-3 protein (Uniprot: P23771)

Localization

Nuclear

Marker

Breast and Urothelial Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14267)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for GATA-3 Antibody (GATA3/4550R)

Western Blot: GATA-3 Antibody (GATA3/4550R) [NBP3-13922]

Western Blot: GATA-3 Antibody (GATA3/4550R) [NBP3-13922]

Western Blot: GATA-3 Antibody (GATA3/4550R) [NBP3-13922] - Western blot analysis of MCF-7 cell lysate using GATA-3 antibody (GATA3/4550R). Specific bands were detected for GATA-3 full length (FL) at approx. 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated).
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/4550R) [NBP3-13922]

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/4550R) [NBP3-13922]

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/4550R) [NBP3-13922] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GATA-3 antibody (GATA3/4550R).
Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/4550R) [NBP3-13922]

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/4550R) [NBP3-13922]

Immunohistochemistry-Paraffin: GATA-3 Antibody (GATA3/4550R) [NBP3-13922] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using GATA-3 antibody (GATA3/4550R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Applications for GATA-3 Antibody (GATA3/4550R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: GATA-3

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Alternate Names

GATA3

Gene Symbol

GATA3

UniProt

P23771

Additional GATA-3 Products

Product Documents for GATA-3 Antibody (GATA3/4550R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-3 Antibody (GATA3/4550R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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