GATA-3 Antibody (GATA3/4550R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14267
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # GATA3/4550R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for GATA-3 Antibody (GATA3/4550R) - Azide and BSA Free
Immunogen
Recombinant full-length human GATA-3 protein (Uniprot: P23771)
Localization
Nuclear
Marker
Breast and Urothelial Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13922).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for GATA-3 Antibody (GATA3/4550R) - Azide and BSA Free
GATA-3 Antibody (GATA3/4550R)
Western blot analysis of MCF-7 cell lysate using GATA-3 antibody (GATA3/4550R). Specific bands were detected for GATA-3 full length (FL) at approx. 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated).Applications for GATA-3 Antibody (GATA3/4550R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: GATA-3
Alternate Names
GATA3
Gene Symbol
GATA3
Additional GATA-3 Products
Product Documents for GATA-3 Antibody (GATA3/4550R) - Azide and BSA Free
Product Specific Notices for GATA-3 Antibody (GATA3/4550R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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