GDF-15 Antibody (CL0328)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52917

Novus Biologicals, part of Bio-Techne
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NBP2-52917
NBP2-52917-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0328

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GDF-15 Antibodies »

Product Summary for GDF-15 Antibody (CL0328)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LSLAEASRASFPGPSELHSEDSRFRELRKRYEDLLTRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQL

Epitope

NQSWEDSNTD

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GDF-15 Antibody (CL0328)

Immunohistochemistry-Paraffin: GDF-15 Antibody (CL0328) [NBP2-52917]

Immunohistochemistry-Paraffin: GDF-15 Antibody (CL0328) [NBP2-52917]

Immunohistochemistry-Paraffin: GDF-15 Antibody (CL0328) [NBP2-52917] - Staining in human placenta and skeletal muscle tissues using NBP2-52917 antibody. Corresponding GDF15 RNA-seq data are presented for the same tissues.
Western Blot: GDF-15 Antibody (CL0328) [NBP2-52917]

Western Blot: GDF-15 Antibody (CL0328) [NBP2-52917]

Western Blot: GDF-15 Antibody (CL0328) [NBP2-52917] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GDF15 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: GDF-15 Antibody (CL0328) [NBP2-52917]

Immunocytochemistry/ Immunofluorescence: GDF-15 Antibody (CL0328) [NBP2-52917]

Immunocytochemistry/Immunofluorescence: GDF-15 Antibody (CL0328) [NBP2-52917] - Staining of A549 cells using the Anti-GDF15 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
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Applications for GDF-15 Antibody (CL0328)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GDF-15

Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.

GDF15 antibodies are useful for studies on inflamation and immune system research.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Gene Symbol

GDF15

Additional GDF-15 Products

Product Documents for GDF-15 Antibody (CL0328)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GDF-15 Antibody (CL0328)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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