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GDF-15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81050

Novus Biologicals, part of Bio-Techne
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NBP1-81050
NBP1-81050-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSLAEASRASFPGPSELHSEDSRFRELRKRYEDLLTRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GDF-15 Antibody

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050]

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050]

Immunohistochemistry-Paraffin: GDF-15 Antibody [NBP1-81050] - Analysis in human placenta and cervix, uterine tissues using NBP1-81050 antibody. Corresponding GDF-15 RNA-seq data are presented for the same tissues.
Western Blot: GDF-15 Antibody [NBP1-81050]

Western Blot: GDF-15 Antibody [NBP1-81050]

Western Blot: GDF-15 Antibody [NBP1-81050] - Analysis in human cell line BEWO.
Immunocytochemistry/ Immunofluorescence: GDF-15 Antibody [NBP1-81050]

Immunocytochemistry/ Immunofluorescence: GDF-15 Antibody [NBP1-81050]

Immunocytochemistry/Immunofluorescence: GDF-15 Antibody [NBP1-81050] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus.

Applications for GDF-15 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

Reactivity reported in (PMID: 26114631).

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GDF-15

Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.

GDF15 antibodies are useful for studies on inflamation and immune system research.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Entrez Gene IDs

9518 (Human)

Gene Symbol

GDF15

UniProt

Additional GDF-15 Products

Product Documents for GDF-15 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GDF-15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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