GFP Antibody [Biotin]

Immunofluorescent Staining of Mouse Cells Using Biotin Conjugated GFP Antibody

IF analysis of GFP in mouse aorta, liver, brain, and lung cells. Image courtesy of product review by Laura Shankman.

Immunohistochemical Detection of GFP in Free Floating Mouse Brain

Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40um. Primary antibody: Goat anti-GFP was used at 1:500 dilution in free floating IHC to detect GFP. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Immunofluorescent Staining of Mouse Pancreas Using Biotin Conjugated GFP Antibody

Staining of transgenic mouse pancreas, expressing GFP in beta cells. Image courtesy of product review by Dr. Yves Heremans of Vrije Universiteit Brussel.

Immunohistochemical Staining of Drosophila Central Nervous System Tissue Using Biotin Conjugated GFP Antibody

Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-GFP antibody at a 1:1,000 for 1 h at RT. Secondary antibody: AlexaFluor 488 conjugated anti-Goat antibody at 1:300 for 45 min at RT. Panel A: shows a lateral view (ventral left). Panels B and C: shows ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up. Staining: tau-GFP cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) as green fluorescent signal.

Detection of Purified GFP in ELISA Using Biotin Conjugated Antibody

ELISA Results of GFP antibody [Biotin] tested against purified GFP protein. Each well was coated in duplicate with 10ug/mL of GFP protein. The starting dilution of antibody was 5ug/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer and SA-HRP conjugated.