HEK293 human embryonic kidney cell line transfected with eGFP was stained with either (A) Mouse Anti-GFP APC-conjugated Monoclonal Antibody (Catalog # IC42401A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for GFP APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with eGFP was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfish Aequorea victoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in length. It is a globular monomer that has a tendency to dimerize. The monomer has the shape of a beta-barrel with a chromophore (aa 65-67) containing alpha-helix running up its center. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. This form expresses faster and is 18-fold brighter than native GFP; excitation peaks at 395 nm and emission at 508 nm.

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

UniProt

P42212

Additional GFP Products

Product Documents for GFP APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for GFP APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

GFP APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC42401A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GFP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for GFP APC-conjugated Antibody

Detection of GFP in HEK293 Human Cell Line Transfected with eGFP by Flow Cytometry.