GINS4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82748

Novus Biologicals, part of Bio-Techne
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NBP1-82748
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GINS4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MTEEVDFLGQDSDGGSEEVVLTPAELIERLEQAWMNEKFAPELLESKPEIVECVMEQLEHMEE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GINS4 Antibody

Western Blot: GINS4 Antibody [NBP1-82748]

Western Blot: GINS4 Antibody [NBP1-82748]

Western Blot: GINS4 Antibody [NBP1-82748] - Analysis in control (vector only transfected HEK293T lysate) and GINS4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: GINS4 Antibody [NBP1-82748]

Immunocytochemistry/ Immunofluorescence: GINS4 Antibody [NBP1-82748]

Immunocytochemistry/Immunofluorescence: GINS4 Antibody [NBP1-82748] - Staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GINS4 Antibody [NBP1-82748]

Immunohistochemistry-Paraffin: GINS4 Antibody [NBP1-82748]

Immunohistochemistry-Paraffin: GINS4 Antibody [NBP1-82748] - Staining of human kidney shows moderate cytopolasmic positivity in tubular cells.

Applications for GINS4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GINS4

The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), andPsf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication inyeast and Xenopus egg extracts (Ueno et al., 2005 (PubMed 16287864)). See GINS1 for additional information about theGINS complex.(supplied by OMIM)

Alternate Names

DNA replication complex GINS protein SLD5, GINS complex subunit 4, GINS complex subunit 4 (Sld5 homolog), SLD5 homolog, SLD5MGC14799

Gene Symbol

GINS4

Additional GINS4 Products

Product Documents for GINS4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GINS4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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