GLI-1 Antibody (1B9F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52475

Novus Biologicals, part of Bio-Techne
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NBP2-52475
NBP2-52475-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B9F8

Format

BSA Free

Concentration

1 mg/ml

View all GLI-1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human GLI-1 (AA: 284-449) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLI-1 Antibody (1B9F8) - BSA Free

Western Blot: GLI-1 Antibody (1B9F8)BSA Free [NBP2-52475]

Western Blot: GLI-1 Antibody (1B9F8)BSA Free [NBP2-52475]

Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]

Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]

Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]

Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) - BSA Free [NBP2-52475]

Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of esophageal cancer tissues using GLI1 mouse mAb with DAB staining.
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Applications for GLI-1 Antibody (1B9F8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLI-1

Gli1 was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that GLI is expressed in embryonal carcinoma cells but not in most adult tissue. GLI has been localized to 12q13-q14.3 by Southern blot analysis. On mouse the gene is located on chromosome 10. In mice, 3 zinc finger transcription factors, Gli-1, Gl-i2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli1 expression is central and probably sufficient for tumor development in humans.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Alternate Names

GLI1

Gene Symbol

GLI1

Additional GLI-1 Products

Product Documents for GLI-1 Antibody (1B9F8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GLI-1 Antibody (1B9F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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