GLI-1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-600
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Canine
Cited:
Human, Mouse, Porcine, Canine
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), ELISA, Flow (Intracellular), Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
Chemotaxis, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide made toward the C-terminus portion of the human GLI-1 protein (between residues 800-850)
Reactivity Notes
Canine reactivity reported from a verified customer review.
Localization
Cytoplasmic and Nuclear
Specificity
No reaction occurs with human or mouse Gli2 or Gli3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GLI-1 Antibody - BSA Free
Knockdown Validated: GLI-1 Antibody - BSA Free [NB600-600]
Knockdown Validated: GLI-1 Antibody - BSA Free [NB600-600] - GLI-1 Antibody [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo and in vitro. (E) IHC staining for Ki-67, -SMA, Gli-1 and LYVE1 in tumour sections from mice in the two groups. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600]
Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600]
Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.Applications for GLI-1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
ELISA
1:500 - 1:2000
Flow (Intracellular)
1-2 ug/million cells
Immunoblotting
1 - 2 ug/ml
Immunocytochemistry/ Immunofluorescence
1 ug/ml
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1 - 2 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID:35226587) Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Flow was reported in a customer review. IHC-P reported from a verified customer review. Use in immunoblotting reported in scientific literature (PMID: 28167297). Use in FLOW reported in scientific literature (PMID: 26238488)..
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB600-600 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GLI-1
Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Alternate Names
GLI1
Gene Symbol
GLI1
UniProt
Additional GLI-1 Products
Product Documents for GLI-1 Antibody - BSA Free
Product Specific Notices for GLI-1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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