GLI-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24662

Novus Biologicals, part of Bio-Techne
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NBP2-24662
NBP2-24662SS
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Equine (%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GLI-1 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 940-990 of human GLI1 was used as the immunogen for this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GLI-1 Antibody

Western Blot: GLI-1 Antibody [NBP2-24662]

Western Blot: GLI-1 Antibody [NBP2-24662]

Western Blot: GLI-1 Antibody [NBP2-24662] - Analysis of human kidney lysate using 1) pre-immune sera at 1:5000 dilution and 2) GLI-1 antibody at 1 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody [NBP2-24662]

Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody [NBP2-24662]

Immunocytochemistry/Immunofluorescence: GLI-1 Antibody [NBP2-24662] - staining of GLI-1 at 10 ug/ml. Top panel: C666-1 malignant human nasopharyngel carcinoma cell line. Bottom panel: OKF6 tert-1 human oral epithelial cell line. GLI-1 was more highly expressed in the C666-1 than in the OKF6 tert-1 cells. See application notes for protocol details.
Immunohistochemistry-Paraffin: GLI-1 Antibody [NBP2-24662]

Immunohistochemistry-Paraffin: GLI-1 Antibody [NBP2-24662]

Immunohistochemistry-Paraffin: GLI-1 Antibody [NBP2-24662] - Formalin-fixed, paraffin-embedded human brain tissue stained with an isotype control antibody (top) and GLI-1 antibody (bottom) at 5 ug/ml.

Applications for GLI-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-3 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.1 ml PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GLI-1

GLI1 is a protein originally isolated in human glioblastoma. The GLI proteins are the effectors of Sonic Hedgehog (Hh) signaling and have been shown to be involved in cell-fate determination, proliferation and patterning in many cell types and most organs during embryo development. GLI1 was originally isolated from a glioma tumour and has been found to be up regulated in many tumors including muscle, brain and skin tumors such as Basal cell carcinoma (BCC). Shh and the Gli genes are normally expressed in hair follicles, and skin tumours expressing GLI1 may arise from hair follicles. The level of GLI1 expression correlates with the tumor grade in bone and soft tissue sarcomas. GLI1 expression can be cytoplasm and or nucleus. The GLI1 protein is tethered in the cytoplasm by binding to SUFU. Activation and translocation of the GLI1 protein to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is also promoted by interaction with ZIC1.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Alternate Names

GLI1

Gene Symbol

GLI1

Additional GLI-1 Products

Product Documents for GLI-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GLI-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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