Glucagon Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23954

Novus Biologicals, part of Bio-Techne
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NBP3-23954
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glucagon Antibodies »

Product Specifications

Immunogen

Glucagon antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Glucagon.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, chimpanzee, and bonobo.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Glucagon Antibody

Glucagon Antibody Immunoprecipitation: Glucagon Antibody [NBP3-23954] -

Immunoprecipitation: Glucagon Antibody [NBP3-23954] -

Immunoprecipitation: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: MCF7 cells. Fixative: 100% Methanol. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.
Glucagon Antibody ELISA: Glucagon Antibody [NBP3-23954] -

ELISA: Glucagon Antibody [NBP3-23954] -

ELISA: Glucagon Antibody [NBP3-23954] - ELISA Results of Glucagon Antibody. Each well was coated with 1µg of conjugate. The starting concentration of antibody in the dilution series was 5 µg/ml. The titer is 1:34,800 Glucagon - Free peptide [Green Line], 1:47200 Glucagon Standard - BSA conjugated [Blue Line], and 1:20,500 Glucagon - BSA conjugated [Purple Line]. Each point on the Y-axis represents a 3-fold dilution. 3% Fish Gel, HRP conjugated Goat anti-Rabbit IgG (H&L), and TMB substrate were used for detection.
Glucagon Antibody Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] -

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] -

Immunocytochemistry/ Immunofluorescence: Glucagon Antibody [NBP3-23954] - Immunofluorescence of Glucagon Antibody. Cell Line: NIH/3T3 cells. Fixative: 100% Methanol. Permeabilization: Triton X-100. Primary Antibody: Anti-Glucagon at 15µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG DyLight™488 at 5µL/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A). DAPI. (B). Anti-Glucagon + DyLight™488 secondary. (C). Merge A+B. (D). secondary only. Localization expected: Cytoplasm.
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Applications for Glucagon Antibody

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Tested in ELISA, IF, and Western Blot. Although not tested, this antibody is suitable in immunohistochemistry. Expect a band approximately ~20.9 kDa corresponding to the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

N/A

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glucagon

The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)

Alternate Names

GCG, GRPP

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glucagon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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