Glypican 3 Antibody (1G12) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48396
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (1G12) - IHC-Prediluted [NBP2-48396]](https://resources.bio-techne.com/images/products/Glypican-3-Antibody-1G12-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48396-img0001.jpg "Immunohistochemistry-Paraffin: Glypican 3 Antibody (1G12) - IHC-Prediluted [NBP2-48396]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1G12
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Glypican 3 Antibody (1G12) - IHC-Prediluted
Immunogen
A recombinant fragment containing amino acids 511-580 of human Glypican 3 (Uniprot: P51654)
Localization
Cytoplasmic
Specificity
Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
Marker
Hepatocellular Carcinoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Glypican 3 Antibody (1G12) - IHC-Prediluted
Immunohistochemistry-Paraffin: Glypican 3 Antibody (1G12) - IHC-Prediluted [NBP2-48396]
Immunohistochemistry-Paraffin: Glypican 3 Antibody (1G12) - IHC-Prediluted [NBP2-48396] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma (HCC) stained with Glypican-3 Monoclonal Antibody (1G12)Applications for Glypican 3 Antibody (1G12) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Glypican 3
Alternate Names
GPC3
Gene Symbol
GPC3
Additional Glypican 3 Products
Product Documents for Glypican 3 Antibody (1G12) - IHC-Prediluted
Product Specific Notices for Glypican 3 Antibody (1G12) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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