Glypican 3 Antibody (7M2X9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15428

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15428
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7M2X9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glypican 3 Antibodies »

Product Summary for Glypican 3 Antibody (7M2X9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-211 of human Glypican 3 (P51654). PGQAQPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glypican 3 Antibody (7M2X9)

Western Blot: Glypican 3 Antibody (7M2X9) [NBP3-15428]

Western Blot: Glypican 3 Antibody (7M2X9) [NBP3-15428]

Western Blot: Glypican 3 Antibody (7M2X9) [NBP3-15428] - Western blot analysis of extracts of HepG2 cells, using Glypican 3 (GPC3) Rabbit mAb (NBP3-15428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (7M2X9) [NBP3-15428]

Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (7M2X9) [NBP3-15428]

Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (7M2X9) [NBP3-15428] - Immunofluorescence analysis of HepG2 cells using Glypican 3 (GPC3) Rabbit mAb (NBP3-15428) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Glypican 3 Antibody (7M2X9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glypican 3

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Alternate Names

GPC3

Gene Symbol

GPC3

Additional Glypican 3 Products

Product Documents for Glypican 3 Antibody (7M2X9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glypican 3 Antibody (7M2X9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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