Glypican 3 Antibody (SPM595)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44485

Novus Biologicals, part of Bio-Techne
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NBP2-44485-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM595

Concentration

0.2 mg/ml

View all Glypican 3 Antibodies »

Product Summary for Glypican 3 Antibody (SPM595)

Immunogen

A recombinant fragment containing amino acids 511-580 of human Glypican 3 (Uniprot: P51654)

Localization

Cytoplasmic

Specificity

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

Marker

Hepatocellular Carcinoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47761)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Glypican 3 Antibody (SPM595)

Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (SPM595) [NBP2-44485]

Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (SPM595) [NBP2-44485]

Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (SPM595) [NBP2-44485] - ICC/IF analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) [NBP2-44485]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) [NBP2-44485]

Immunohistochemistry-Paraffin: Glypican 3 Antibody (SPM595) [NBP2-44485] - Human Melanoma stained with Glypican-3 Monoclonal Antibody (SPM595)
Flow Cytometry: Glypican 3 Antibody (SPM595) [NBP2-44485]

Flow Cytometry: Glypican 3 Antibody (SPM595) [NBP2-44485]

Flow Cytometry: Glypican 3 Antibody (SPM595) [NBP2-44485] - Flow Cytometric Analysis of PFA-fixed HepG2 cells using Glypican 3 Antibody (SPM595) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).
View 1 more images

Applications for Glypican 3 Antibody (SPM595)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Glypican 3

The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Alternate Names

GPC3

Gene Symbol

GPC3

UniProt

P51654

Additional Glypican 3 Products

Product Documents for Glypican 3 Antibody (SPM595)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glypican 3 Antibody (SPM595)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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