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GPR55 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55498

Novus Biologicals, part of Bio-Techne
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NB110-55498

Key Product Details

Species Reactivity

Validated:

Human, Canine

Cited:

Canine, Equine

Predicted:

Monkey (95%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR55.

Epitope

Third cytoplasmic loop of human

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (95%), Marmoset (95%), Bovine (85%), Mouse (80%), Equine (80%), Porcine (80%). Canine reactivity reported from a verified customer review.

Specificity

Human GPR55. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for GPR55 Antibody - BSA Free

Immunohistochemistry: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry: GPR55 Antibody [NB110-55498] - Photomicrographs of cryosections of canine cervical (C8) dorsal root ganglion showing GPR55 (b-f) immunolabeling. (a-c) Arrows indicate the Neurotrace-labeled nuclei of satellite glial cells which showed bright GPR55 immunolabelling (c). White stars indicate unlabeled sensory neurons; open stars indicate empty spaces in which sensory neurons were no more evident. (d-f) White arrows indicate satellite glial cells which co-expressed bright GPR55- (d) and glial fibrillary acidic protein (GFAP) immunoreactivity. Stars indicate sensory neurons of different dimension, which expressed faint-to-moderate GPR55 immunoreactivity. Open arrow indicate autofluorescent pigment. Bar: 50 um. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.
Western Blot: GPR55 AntibodyBSA Free [NB110-55498]

Western Blot: GPR55 AntibodyBSA Free [NB110-55498]

Western Blot: GPR55 Antibody [NB110-55498] - Representative image of Western blots (WB) analysis showing the specificity of the primary antibody rabbit anti-G protein-coupled receptor 55 (GPR55). The antibody revealed a single band of expected molecular weight (~40 kDa). The images of the different immunoblots were slightly adjusted in brightness and contrast to match their backgrounds. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fvets.2019.00313/full) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry-Paraffin: GPR55 Antibody - BSA Free [NB110-55498]

Immunohistochemistry-Paraffin: GPR55 Antibody [NB110-55498] - Analysis of anti-GPR55 antibody with human pancreas, islet of langerhans.

Applications for GPR55 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml
Application Notes
Use in WB reported in scientific literature (PMID:31608295). GPR55 antibody validated for IHC-F from a verified customer review..

Reviewed Applications

Read 1 review rated 5 using NB110-55498 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: GPR55

G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries.

Long Name

G-Protein-Coupled Receptor 55

Alternate Names

Gm218

Entrez Gene IDs

9290 (Human)

Gene Symbol

GPR55

UniProt

Additional GPR55 Products

Product Documents for GPR55 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPR55 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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