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GRAP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83399

Novus Biologicals, part of Bio-Techne
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NBP1-83399
NBP1-83399-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TNSISRQKQIFLRDRTREDQGHRGNSLDRRSQGGPHLSGAVGEEIRPSMNRKLSDHPPTLPLQQHQHQPQPPQYAPAPQQLQQPPQQRYLQHHHFHQERRGGSLDINDGHCGTGLGSEMNAALMHRRHTDPVQLQAAGRVRWARALYDFE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRAP2 Antibody

Western Blot: GRAP2 Antibody [NBP1-83399]

Western Blot: GRAP2 Antibody [NBP1-83399]

Western Blot: GRAP2 Antibody [NBP1-83399] - Analysis in control (vector only transfected HEK293T lysate) and GRAP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: GRAP2 Antibody [NBP1-83399]

Immunohistochemistry-Paraffin: GRAP2 Antibody [NBP1-83399]

Immunohistochemistry-Paraffin: GRAP2 Antibody [NBP1-83399] - Staining in human tonsil and cerebral cortex tissues using anti-GRAP2 antibody. Corresponding GRAP2 RNA-seq data are presented for the same tissues.
Western Blot: GRAP2 Antibody [NBP1-83399]

Western Blot: GRAP2 Antibody [NBP1-83399]

Western Blot: GRAP2 Antibody [NBP1-83399] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4

Applications for GRAP2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRAP2

GRAP2 encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.

Long Name

GRB2-related Adaptor Protein 2

Alternate Names

GADS, GRB2L, GRBLG, GRBX, Grf40, GRID, GRPL, Mona

Gene Symbol

GRAP2

Additional GRAP2 Products

Product Documents for GRAP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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