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GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15149

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15149

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4A5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-GSK-3 alpha (Y216 + Y279). UniProt ID (P49840).

Modification

p Tyr216, p Tyr279

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5)

Immunohistochemistry: GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5) [NBP3-15149] - IHC image of GSK-3 alpha antibody diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minute at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Applications for GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: GSK-3 alpha

Glycogen synthases kinase 3 alpha (GSK3-alpha) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-alpha activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-alpha by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-alpha activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4).

Long Name

Glycogen Synthase Kinase 3 alpha

Alternate Names

GSK3 alpha, GSK3A

Gene Symbol

GSK3A

Additional GSK-3 alpha Products

Product Documents for GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GSK-3 alpha [p Tyr216, p Tyr279] Antibody (4A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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