GSK-3 alpha Antibody (4I10Q9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15639

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15639-100ul
NBP3-15639-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4I10Q9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GSK-3 alpha Antibodies »

Product Summary for GSK-3 alpha Antibody (4I10Q9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GSK-3 alpha (P49840). MSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGGPGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPGGSGGGGSGGPGAGTSFPPPGVKLGRDSGKV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GSK-3 alpha Antibody (4I10Q9)

Western Blot: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639]

Western Blot: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639]

Western Blot: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639] - Western blot analysis of extracts of Mouse brain, using GSK-3 alpha antibody (NBP3-15639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639]

Western Blot: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639]

Western Blot: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639] - Western blot analysis of extracts of Rat lung, using GSK-3 alpha antibody (NBP3-15639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Knockout Validated: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639]

Knockout Validated: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639]

Knockout Validated: GSK-3 alpha Antibody (4I10Q9) [NBP3-15639] - Western blot analysis of extracts from normal (control) and GSK-3 alpha knockout (KO) HeLa cells, using GSK-3 alpha antibody (NBP3-15639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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Applications for GSK-3 alpha Antibody (4I10Q9)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GSK-3 alpha

Glycogen synthases kinase 3 alpha (GSK3-alpha) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-alpha activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-alpha by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-alpha activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4).

Long Name

Glycogen Synthase Kinase 3 alpha

Alternate Names

GSK3 alpha, GSK3A

Gene Symbol

GSK3A

Additional GSK-3 alpha Products

Product Documents for GSK-3 alpha Antibody (4I10Q9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GSK-3 alpha Antibody (4I10Q9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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