GSK-3 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81943

Novus Biologicals, part of Bio-Techne
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NB100-81943
NB100-81943-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GSK-3 alpha Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human GSK-3 alpha around amino acids 19~23 (T-S-S-F-A). IP data from customer review.

Specificity

Detects endogenous levels of total GSK-3 alpha protein. Does not detect GSK-3 beta protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSK-3 alpha Antibody

Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] -

Immunocytochemistry/Immunofluorescence: GSK-3 alpha Antibody [NB100-81943] - Staining of methanol-fixed MCF7 cells NB100-81943

Immunohistochemistry-Paraffin: GSK-3 alpha Antibody [NB100-81943] -

Immunohistochemistry-Paraffin: GSK-3 alpha Antibody [NB100-81943] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-81943 (left) or the same antibody preincubated with blocking peptide (right).

Western Blot: GSK-3 alpha Antibody [NB100-81943] -

Western Blot: GSK-3 alpha Antibody [NB100-81943] - Analysis of extracts from 293 cells using NB100-81943
View 1 more images

Applications for GSK-3 alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100~1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-81943 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSK-3 alpha

Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.

Long Name

Glycogen Synthase Kinase 3 alpha

Alternate Names

GSK3 alpha, GSK3A

Entrez Gene IDs

2931 (Human); 50686 (Rat)

Gene Symbol

GSK3A

UniProt

P49840

Additional GSK-3 alpha Products

Product Documents for GSK-3 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GSK-3 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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