GSK-3 alpha Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15579

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GSK-3 alpha Antibodies »

Product Summary for GSK-3 alpha Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-483 of human GSK-3 alpha (NP_063937.2). HSFFDELRCLGTQLPNNRPLPPLFNFSAGELSIQPSLNAILIPPHLRSPAGTTTLTPSSQALTETPTSSDWQSTDATPTLTNSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GSK-3 alpha Antibody - Azide and BSA Free

Western Blot: GSK-3 alpha AntibodyAzide and BSA Free [NBP3-15579]

Western Blot: GSK-3 alpha AntibodyAzide and BSA Free [NBP3-15579]

Western Blot: GSK-3 alpha Antibody [NBP3-15579] - Western blot analysis of extracts of various cell lines, using GSK-3 alpha antibody (NBP3-15579) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Knockout Validated: GSK-3 alpha Antibody - Azide and BSA Free [NBP3-15579]

Knockout Validated: GSK-3 alpha Antibody - Azide and BSA Free [NBP3-15579]

Knockout Validated: GSK-3 alpha Antibody [NBP3-15579] - Western blot analysis of extracts from normal (control) and GSK-3 alpha knockout (KO) HeLa cells, using GSK-3 alpha antibody (18655) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Applications for GSK-3 alpha Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GSK-3 alpha

Glycogen synthases kinase 3 alpha (GSK3-alpha) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-alpha activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-alpha by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-alpha activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4).

Long Name

Glycogen Synthase Kinase 3 alpha

Alternate Names

GSK3 alpha, GSK3A

Gene Symbol

GSK3A

Additional GSK-3 alpha Products

Product Documents for GSK-3 alpha Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GSK-3 alpha Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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