GSK-3 beta Antibody (GT1619)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13691

Novus Biologicals, part of Bio-Techne
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NBP3-13691
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT1619

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GSK-3 beta Antibodies »

Product Summary for GSK-3 beta Antibody (GT1619)

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GSK-3 beta. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GSK-3 beta Antibody (GT1619)

Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691]

Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691]

Western Blot: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (GT1619) [NBP3-13691]

Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (GT1619) [NBP3-13691]

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (GT1619) [NBP3-13691] - GSK-3 beta antibody detects GSK-3 beta protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK-3 beta stained by GSK-3 beta antibody (NBP3-13691) diluted at 1:500.
Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691]

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691]

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (GT1619) [NBP3-13691] - Rat brain. GSK-3 beta stained by GSK-3 beta antibody [GT1619] (NBP3-13691) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for GSK-3 beta Antibody (GT1619)

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
ICC/IF, IHC-P-Assay dependent.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSK-3 beta

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Long Name

Glycogen Synthase Kinase 3 beta

Alternate Names

GSK3 beta, GSK3B

Gene Symbol

GSK3B

Additional GSK-3 beta Products

Product Documents for GSK-3 beta Antibody (GT1619)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GSK-3 beta Antibody (GT1619)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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