GSK-3 beta [p Tyr279, p Tyr216] Antibody

Name: GSK-3 beta [p Tyr279, p Tyr216] Antibody
Brand: Novus Biologicals
SKU: NB100-81946

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81946

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Mammal

Cited:

Human, Mouse, Mammal

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GSK-3 beta Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human GSK-3 beta around the phosphorylation site of tyrosine 279/216 (V-S-YP-I-C).

Reactivity Notes

Use in Mammal reported in scientific literature (PMID:33413691)

Modification

p Tyr216, p Tyr279

Specificity

Detects endogenous levels of GSK-3 beta only when phosphorylated at tyrosine 216/279.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSK-3 beta [p Tyr279, p Tyr216] Antibody

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946]

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Analysis of extracts of various cell lines,using GSK-3 beta (Phospho-Tyr279/216) Antibody

Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946]

Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Methanol-fixed MEF cells.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81946 (left) or the same antibody preincubated with blocking peptide (right).

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946]

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Analysis of extracts from 293 cells untreated or treated with PMA using GSK-3 beta (P-Tyr216) antibody.

Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946]

Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Staining of Methanol-fixed Hela cells.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, NB100-81946 was diluted at 1: 200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, NB100-81946 was diluted at 1:200 (4C, overnight). 2, Sodium citrate pH 6. 0 was used for antibody retrieval (>98C, 20min). 3, Secondary antibody was diluted at 1: 200 (room temperature, 30min). Negative control was used by secondary antibody only.

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Western blot analysis of GSK-3 beta [p Tyr279, p Tyr216] expression in mouse testis (A), rat testis (B), rat spleen (C) whole cell lysates.

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] - Western blot analysis of extracts from 293 cells untreated or treated with PMA using NB100-81946

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Applications for GSK-3 beta [p Tyr279, p Tyr216] Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:100-1:200

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:1000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol

Preservative

0.01% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSK-3 beta

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation.

Long Name

Glycogen Synthase Kinase 3 beta

Alternate Names

GSK3 beta, GSK3B

Entrez Gene IDs

2932 (Human); 56637 (Mouse); 84027 (Rat)

Gene Symbol

GSK3B

UniProt

P49841

Additional GSK-3 beta Products

Product Documents for GSK-3 beta [p Tyr279, p Tyr216] Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for GSK-3 beta [p Tyr279, p Tyr216] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

GSK-3 beta [p Tyr279, p Tyr216] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81946

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all GSK-3 beta Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Modification

Specificity

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for GSK-3 beta [p Tyr279, p Tyr216] Antibody

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946]

Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946]

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946]

Immunocytochemistry/ Immunofluorescence: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946]

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Immunohistochemistry-Paraffin: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Western Blot: GSK-3 beta [p Tyr279, p Tyr216] Antibody [NB100-81946] -

Applications for GSK-3 beta [p Tyr279, p Tyr216] Antibody

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: GSK-3 beta

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional GSK-3 beta Products

Product Documents for GSK-3 beta [p Tyr279, p Tyr216] Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for GSK-3 beta [p Tyr279, p Tyr216] Antibody