H4/n Antibody (3E7H2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52486

Novus Biologicals, part of Bio-Techne
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NBP2-52486
NBP2-52486-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3E7H2

Format

BSA Free

Concentration

1 mg/ml

View all H4/n Antibodies »

Product Summary for H4/n Antibody (3E7H2) - BSA Free

Immunogen

Synthesized peptide of human H4/n (AA: GGAKRHRK(Me)VLRDNIQ).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

11.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for H4/n Antibody (3E7H2) - BSA Free

Western Blot: H4/n Antibody (3E7H2)BSA Free [NBP2-52486]

Western Blot: H4/n Antibody (3E7H2)BSA Free [NBP2-52486]

Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Immunocytochemistry/ Immunofluorescence: H4/n Antibody (3E7H2) - BSA Free [NBP2-52486]

Immunocytochemistry/ Immunofluorescence: H4/n Antibody (3E7H2) - BSA Free [NBP2-52486]

Immunocytochemistry/Immunofluorescence: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: H4/n Antibody (3E7H2) - BSA Free [NBP2-52486]

Immunohistochemistry-Paraffin: H4/n Antibody (3E7H2) - BSA Free [NBP2-52486]

Immunohistochemistry-Paraffin: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
View 4 more images

Applications for H4/n Antibody (3E7H2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: H4/n

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. H4/N is a103 amino acid long, 11 kDA protein encoded by the HIST2H4A gene found on chromosome 1. HIST2H4A is one of four histone genes in this specific cluster that are duplicated, representing the centromeric copy. HIST2H4A is involved in type II interferon signaling, development notch signaling pathway, transcription Sin3 and NuRD in transcription regulation, and telomere maintenance through interactions with genes HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, and HIST1H3F. It has been investigated for its role in lymphoma, carcinoma, leukemia, herpes simplex, thyroiditis, and lupus.

Alternate Names

FO108, H4, H4 histone family, member N, H4 histone, family 2, H4/A, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/n, H4/O, H4F2H4/B, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FNH4/C, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4B, HIST4H4, histone 2, H4, histone 2, H4a, histone cluster 2, H4a, histone H4, histone IV, family 2

Gene Symbol

HIST2H4A

Additional H4/n Products

Product Documents for H4/n Antibody (3E7H2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for H4/n Antibody (3E7H2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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